biotin tagged dna probes Search Results


biotin tagged cytor antisense dna probes  (LGC Biosearch)
90
LGC Biosearch biotin tagged cytor antisense dna probes
Increase of <t>CYTOR</t> promotes invasion and tumorigenicity of tumor budding cells in HNSCC. a) Representative images of CYTOR ISH staining in ANCE, tumor center and TB in primary HNSCC, and lymph node metastatic carcinoma (LNM). Scale bar, 100 µm. b–d) Quantification of total b), cytoplasmic c), and nuclear d) CYTOR expression in adjacent non‐cancerous epithelium, tumor center, TB and LNM. * p < 0.05, ** p < 0.01, and *** p < 0.001 by one‐way ANOVA. ns, not significant. e) Detection of expression and subcellular localization of CYTOR in HNSCC cell lines SCC1, HN6, CAL33 and SCC15 by fluorescence in situ hybridization (FISH) and Immunofluorescence (IF) experiments, CYTOR (red) and actin (green). Scale bar, 20 µm. f,g) Evaluation of capacities of tumor sphere formation in HNSCC cells with depletion f) or overexpression g) of CYTOR by extreme limited dilution assay (ELDA), and the frequency and p ‐value computed by ELDA software. h,i) Representative images and evaluation of invasion in HNSCC cells with depletion h) and overexpression i) of CYTOR by transwell assay. Scale bar, 200 µm. ** p < 0.01 and *** p < 0.001 by one‐way ANOVA (h). ** p < 0.01 by Student's t ‐test (i).
Biotin Tagged Cytor Antisense Dna Probes, supplied by LGC Biosearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+tagged+dna+probes/biotin+tagged+cytor+antisense+dna+probes/pmc10811474-306-0-11
Average 90 stars, based on 1 article reviews
biotin tagged cytor antisense dna probes - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Increase of CYTOR promotes invasion and tumorigenicity of tumor budding cells in HNSCC. a) Representative images of CYTOR ISH staining in ANCE, tumor center and TB in primary HNSCC, and lymph node metastatic carcinoma (LNM). Scale bar, 100 µm. b–d) Quantification of total b), cytoplasmic c), and nuclear d) CYTOR expression in adjacent non‐cancerous epithelium, tumor center, TB and LNM. * p < 0.05, ** p < 0.01, and *** p < 0.001 by one‐way ANOVA. ns, not significant. e) Detection of expression and subcellular localization of CYTOR in HNSCC cell lines SCC1, HN6, CAL33 and SCC15 by fluorescence in situ hybridization (FISH) and Immunofluorescence (IF) experiments, CYTOR (red) and actin (green). Scale bar, 20 µm. f,g) Evaluation of capacities of tumor sphere formation in HNSCC cells with depletion f) or overexpression g) of CYTOR by extreme limited dilution assay (ELDA), and the frequency and p ‐value computed by ELDA software. h,i) Representative images and evaluation of invasion in HNSCC cells with depletion h) and overexpression i) of CYTOR by transwell assay. Scale bar, 200 µm. ** p < 0.01 and *** p < 0.001 by one‐way ANOVA (h). ** p < 0.01 by Student's t ‐test (i).

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: Increase of CYTOR promotes invasion and tumorigenicity of tumor budding cells in HNSCC. a) Representative images of CYTOR ISH staining in ANCE, tumor center and TB in primary HNSCC, and lymph node metastatic carcinoma (LNM). Scale bar, 100 µm. b–d) Quantification of total b), cytoplasmic c), and nuclear d) CYTOR expression in adjacent non‐cancerous epithelium, tumor center, TB and LNM. * p < 0.05, ** p < 0.01, and *** p < 0.001 by one‐way ANOVA. ns, not significant. e) Detection of expression and subcellular localization of CYTOR in HNSCC cell lines SCC1, HN6, CAL33 and SCC15 by fluorescence in situ hybridization (FISH) and Immunofluorescence (IF) experiments, CYTOR (red) and actin (green). Scale bar, 20 µm. f,g) Evaluation of capacities of tumor sphere formation in HNSCC cells with depletion f) or overexpression g) of CYTOR by extreme limited dilution assay (ELDA), and the frequency and p ‐value computed by ELDA software. h,i) Representative images and evaluation of invasion in HNSCC cells with depletion h) and overexpression i) of CYTOR by transwell assay. Scale bar, 200 µm. ** p < 0.01 and *** p < 0.001 by one‐way ANOVA (h). ** p < 0.01 by Student's t ‐test (i).

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: Staining, Expressing, Fluorescence, In Situ Hybridization, Immunofluorescence, Over Expression, Dilution Assay, Software, Transwell Assay

CYTOR promotes HNSCC tumor growth and metastasis. a) Overexpression of CYTOR promotes the growth of HNSCC orthotropic xenograft formed by CAL33. Scale bar, 1 cm. b) Representative images of HE staining of CAL33 formed xenograft. Scale bar, 1 mm. c) Quantification of tumor volume of HNSCC orthotropic xenograft. * p < 0.05 by Student's t ‐test. d) Representative images of PCK IHC staining in cervical lymph node. Scale bar, 300 µm. Evaluation of the percentage of cervical lymph node with metastasis by Fisher's exact test, * p < 0.05. e) Depletion of CYTOR inhibits the growth of HNSCC orthotropic xenograft formed by SCC1. Scale bar, 1 cm. f) Representative images of HE staining of SCC1 formed orthotropic xenograft. Scale bar, 1 mm. g) Quantification of tumor volume of SCC1 formed orthotropic xenograft. * p < 0.05 and ** p < 0.01 by one‐way ANOVA. h) Representative images of PCK IHC staining in cervical lymph node. Scale bar, 300 µm. * p < 0.05 and ** p < 0.01 by Chi‐square test. i–l) Quantification of CYTOR expression based on ISH staining in HNSCC tissues grouped by with/without cervical lymph node metastasis i), tumor stage (T1, 2 and T3, 4) j), clinical stage (C1, 2 and C3, 4) k) and differentiation degree (well and middle plus poor) l). * p < 0.05, ** p < 0.01, and *** p < 0.001 by Student's t ‐test. m,n) Kaplan‐Meier curves for overall survival m) and disease‐free survival n) of HNSCC patients grouped by the expression of CYTOR based on ISH staining, and difference evaluated by log‐rank test. o) Quantification of CYTOR expression in normal and HNSCC tissues from TCGA database. *** p < 0.001 by Student's t ‐test. p) Expression of CYTOR in 43 paired HNSCC and normal tissues from TCGA database evaluated with Paired t ‐test. q–t) Quantification of CYTOR expression from TCGA database in HNSCC grouped by tumor stage (T1, 2 and T3, 4) q), with/without cervical lymph node metastasis r), clinical stage (C1, 2 and C3, 4) s) and differentiation degree (G1 and G2 plus G3) t). * p < 0.05, ** p < 0.01, and *** p < 0.001 by Student's t ‐test. u,v) Kaplan‐Meier curves and log‐rank test for overall survival u) and disease‐free survival v) of HNSCC patients grouped by the expression of CYTOR from TCGA database.

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: CYTOR promotes HNSCC tumor growth and metastasis. a) Overexpression of CYTOR promotes the growth of HNSCC orthotropic xenograft formed by CAL33. Scale bar, 1 cm. b) Representative images of HE staining of CAL33 formed xenograft. Scale bar, 1 mm. c) Quantification of tumor volume of HNSCC orthotropic xenograft. * p < 0.05 by Student's t ‐test. d) Representative images of PCK IHC staining in cervical lymph node. Scale bar, 300 µm. Evaluation of the percentage of cervical lymph node with metastasis by Fisher's exact test, * p < 0.05. e) Depletion of CYTOR inhibits the growth of HNSCC orthotropic xenograft formed by SCC1. Scale bar, 1 cm. f) Representative images of HE staining of SCC1 formed orthotropic xenograft. Scale bar, 1 mm. g) Quantification of tumor volume of SCC1 formed orthotropic xenograft. * p < 0.05 and ** p < 0.01 by one‐way ANOVA. h) Representative images of PCK IHC staining in cervical lymph node. Scale bar, 300 µm. * p < 0.05 and ** p < 0.01 by Chi‐square test. i–l) Quantification of CYTOR expression based on ISH staining in HNSCC tissues grouped by with/without cervical lymph node metastasis i), tumor stage (T1, 2 and T3, 4) j), clinical stage (C1, 2 and C3, 4) k) and differentiation degree (well and middle plus poor) l). * p < 0.05, ** p < 0.01, and *** p < 0.001 by Student's t ‐test. m,n) Kaplan‐Meier curves for overall survival m) and disease‐free survival n) of HNSCC patients grouped by the expression of CYTOR based on ISH staining, and difference evaluated by log‐rank test. o) Quantification of CYTOR expression in normal and HNSCC tissues from TCGA database. *** p < 0.001 by Student's t ‐test. p) Expression of CYTOR in 43 paired HNSCC and normal tissues from TCGA database evaluated with Paired t ‐test. q–t) Quantification of CYTOR expression from TCGA database in HNSCC grouped by tumor stage (T1, 2 and T3, 4) q), with/without cervical lymph node metastasis r), clinical stage (C1, 2 and C3, 4) s) and differentiation degree (G1 and G2 plus G3) t). * p < 0.05, ** p < 0.01, and *** p < 0.001 by Student's t ‐test. u,v) Kaplan‐Meier curves and log‐rank test for overall survival u) and disease‐free survival v) of HNSCC patients grouped by the expression of CYTOR from TCGA database.

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: Over Expression, Staining, Immunohistochemistry, Expressing

CYTOR controls FOSL1 signaling in HNSCC. a,b) GO analysis for CYTOR governing signaling pathway in SCC1 a) and HN6 b) after depletion of CYTOR . c) GSEA showed that the EMT, mammary stem cell, cancer stemness, and FOSL1 signature were significantly inhibited by depletion of CYTOR in SCC1 and HN6. d) Western‐blot analysis showed that FOSL1 protein levels were downregulated with knockdown of CYTOR and upregulated with overexpression of CYTOR . e) RT‐qPCR showed that FOSL1 transcription was suppressed by depletion of CYTOR in SCC1 and HN6, *** p < 0.001 by one‐way ANOVA. And FOSL1 mRNA was upregulated by overexpression of CYTOR in CAL33 and SCC15, ** p < 0.01 and *** p < 0.001 by Student's t ‐test. f) AP‐1 activities in HNSCC cells with/without CYTOR depletion were evaluated by luciferase reporter assay. *** p < 0.001 by one‐way ANOVA. g) RNA pull‐down and Western blot assay showed that CYTOR physically interacted with FOSL1 in HNSCC cells. h) RIP and RT‐qPCR showed that CYTOR was co‐immunoprecipitated with FOSL1. ** p < 0.01 by Student's t ‐test.

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: CYTOR controls FOSL1 signaling in HNSCC. a,b) GO analysis for CYTOR governing signaling pathway in SCC1 a) and HN6 b) after depletion of CYTOR . c) GSEA showed that the EMT, mammary stem cell, cancer stemness, and FOSL1 signature were significantly inhibited by depletion of CYTOR in SCC1 and HN6. d) Western‐blot analysis showed that FOSL1 protein levels were downregulated with knockdown of CYTOR and upregulated with overexpression of CYTOR . e) RT‐qPCR showed that FOSL1 transcription was suppressed by depletion of CYTOR in SCC1 and HN6, *** p < 0.001 by one‐way ANOVA. And FOSL1 mRNA was upregulated by overexpression of CYTOR in CAL33 and SCC15, ** p < 0.01 and *** p < 0.001 by Student's t ‐test. f) AP‐1 activities in HNSCC cells with/without CYTOR depletion were evaluated by luciferase reporter assay. *** p < 0.001 by one‐way ANOVA. g) RNA pull‐down and Western blot assay showed that CYTOR physically interacted with FOSL1 in HNSCC cells. h) RIP and RT‐qPCR showed that CYTOR was co‐immunoprecipitated with FOSL1. ** p < 0.01 by Student's t ‐test.

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: Western Blot, Knockdown, Over Expression, Quantitative RT-PCR, Luciferase, Reporter Assay, Immunoprecipitation

CYTOR associates with FOSL1 to establish SEs in HNSCC. a) FOSL1 and CYTOR occupancies on genome in SCC1 cells after transfection with control siRNA, FOSL1 siRNA or CYTOR siRNA by ChIP‐seq and ChIRP‐seq. b) The percent of different genomic distribution of FOSL1 and CYTOR peaks on genome in SCC1 by ChIP‐seq analysis. c) Co‐localization of FOSL1 and CYTOR at genome‐wide analyzed by ChIP‐seq and ChIRP‐seq. d) FOSL1‐dependent SEs were identified by ChIP‐seq signal of FOSL1, and CYTOR selectively co‐localized with FOSL1 at FOSL1‐dependent SEs. e) Depletion of FOSL1 or CYTOR dramatically reduced SEs in SCC1 cells based on ChIP‐seq signaling of FOSL1. f,g) The occupancy of FOSL1 and CYTOR at SEs regions of FOSL1 (F) and SNAI2 (G) in SCC1 cells treated with control siRNA, FOSL1 siRNA, or CYTOR siRNA by ChIP‐seq and ChIRP analysis. h) ChIP‐qPCR showed that depletion of CYTOR or FOSL1 by siRNA decreased the recruitment of FOSL1 on the SE regions of FOSL1 and SNAI2 in HNSCC cells. ** p < 0.01 by one‐way ANOVA. i) ChIRP‐qPCR showed that the recruitment of CYTOR on the SE regions of FOSL1 and SNAI2 was significantly reduced in HNSCC cells treated with FOSL1 or CYTOR siRNA. ** p < 0.01 by one‐way ANOVA.

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: CYTOR associates with FOSL1 to establish SEs in HNSCC. a) FOSL1 and CYTOR occupancies on genome in SCC1 cells after transfection with control siRNA, FOSL1 siRNA or CYTOR siRNA by ChIP‐seq and ChIRP‐seq. b) The percent of different genomic distribution of FOSL1 and CYTOR peaks on genome in SCC1 by ChIP‐seq analysis. c) Co‐localization of FOSL1 and CYTOR at genome‐wide analyzed by ChIP‐seq and ChIRP‐seq. d) FOSL1‐dependent SEs were identified by ChIP‐seq signal of FOSL1, and CYTOR selectively co‐localized with FOSL1 at FOSL1‐dependent SEs. e) Depletion of FOSL1 or CYTOR dramatically reduced SEs in SCC1 cells based on ChIP‐seq signaling of FOSL1. f,g) The occupancy of FOSL1 and CYTOR at SEs regions of FOSL1 (F) and SNAI2 (G) in SCC1 cells treated with control siRNA, FOSL1 siRNA, or CYTOR siRNA by ChIP‐seq and ChIRP analysis. h) ChIP‐qPCR showed that depletion of CYTOR or FOSL1 by siRNA decreased the recruitment of FOSL1 on the SE regions of FOSL1 and SNAI2 in HNSCC cells. ** p < 0.01 by one‐way ANOVA. i) ChIRP‐qPCR showed that the recruitment of CYTOR on the SE regions of FOSL1 and SNAI2 was significantly reduced in HNSCC cells treated with FOSL1 or CYTOR siRNA. ** p < 0.01 by one‐way ANOVA.

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: Transfection, Control, ChIP-sequencing, Genome Wide, ChIP-qPCR

CYTOR promotes the formation of FOSL1 phase‐separated condensates. a,b) Representative fluorescence images of phase separated condensates formed by purified FOSL1 protein labeled with Alexa Fluor 488 by in vitro droplet assay, with increasing protein concentration a) and salt concentration b) as indicated. Scale bar, 5 µm. c) Representative fluorescence images of FOSL1 puncta formation in live HNSCC cells transfected with EGFP‐FOSL1 plasmid. Scale bar, 5 µm. d) Time‐lapse fluorescence images showed that FOSL1 droplets underwent fusion event in HNSCC live cells. e) Time‐lapse fluorescence images of FRAP experiment of EGFP‐FOSL1 puncta in live HNSCC cells. Scale bar, 5 µm. f) Quantification of normalized fluorescence intensity of EGFP‐FOSL1 droplets in indicated region of live HNSCC cells with FRAP experiment. g) Fluorescence images showed that mixture of fluorophore‐tagged FOSL1 (10 µ m ) and Cy3‐labeled CYTOR (2 µ m ) formed phase separated condensates containing both FOSL1 and CYTOR in droplet formation assay buffer. Scale bar, 5 µm. h) Fluorescence images of FOSL1 condensates formation at indicated concentration with the presence of CYTOR (1 µ m ), scrambled RNA (1 µ m ) or the absence of RNA. Scale bar, 5 µm. i) Percentage fluorescence fraction of FOSL1 condensates was measured in droplet formation assay at indicated concentration, mixed with CYTOR , scrambled RNA or no RNA. *** p < 0.001 by two‐way ANOVA. j) Time‐lapse fluorescence images of FRAP experiment of EGFP‐FOSL1 puncta in live HNSCC cells transfected with control siRNA and CYTOR siRNA at indicated time. Scale bar, 5 µm. k) Quantification of normalized fluorescence intensity of FOSL1 droplets of live HNSCC cells treated with control siRNA or CYTOR siRNA at indicated time. *** p < 0.001 by two‐way ANOVA.

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: CYTOR promotes the formation of FOSL1 phase‐separated condensates. a,b) Representative fluorescence images of phase separated condensates formed by purified FOSL1 protein labeled with Alexa Fluor 488 by in vitro droplet assay, with increasing protein concentration a) and salt concentration b) as indicated. Scale bar, 5 µm. c) Representative fluorescence images of FOSL1 puncta formation in live HNSCC cells transfected with EGFP‐FOSL1 plasmid. Scale bar, 5 µm. d) Time‐lapse fluorescence images showed that FOSL1 droplets underwent fusion event in HNSCC live cells. e) Time‐lapse fluorescence images of FRAP experiment of EGFP‐FOSL1 puncta in live HNSCC cells. Scale bar, 5 µm. f) Quantification of normalized fluorescence intensity of EGFP‐FOSL1 droplets in indicated region of live HNSCC cells with FRAP experiment. g) Fluorescence images showed that mixture of fluorophore‐tagged FOSL1 (10 µ m ) and Cy3‐labeled CYTOR (2 µ m ) formed phase separated condensates containing both FOSL1 and CYTOR in droplet formation assay buffer. Scale bar, 5 µm. h) Fluorescence images of FOSL1 condensates formation at indicated concentration with the presence of CYTOR (1 µ m ), scrambled RNA (1 µ m ) or the absence of RNA. Scale bar, 5 µm. i) Percentage fluorescence fraction of FOSL1 condensates was measured in droplet formation assay at indicated concentration, mixed with CYTOR , scrambled RNA or no RNA. *** p < 0.001 by two‐way ANOVA. j) Time‐lapse fluorescence images of FRAP experiment of EGFP‐FOSL1 puncta in live HNSCC cells transfected with control siRNA and CYTOR siRNA at indicated time. Scale bar, 5 µm. k) Quantification of normalized fluorescence intensity of FOSL1 droplets of live HNSCC cells treated with control siRNA or CYTOR siRNA at indicated time. *** p < 0.001 by two‐way ANOVA.

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: Fluorescence, Purification, Labeling, In Vitro, Protein Concentration, Concentration Assay, Transfection, Plasmid Preparation, Tube Formation Assay, Control

CYTOR is regulated by FOSL1 in HNSCC. a) FOSL1 occupancies at the locus of CYTOR by FOSL1 ChIP‐seq. b) Knockdown of FOSL1 with siRNA reduced the recruitment of FOSL1 at the promoter of CYTOR in HNSCC cells by ChIP‐qPCR. ** p < 0.01 and *** p < 0.001 by Student's t ‐test. c) Depletion of FOSL1 with siRNA suppressed the expression of CYTOR in HNSCC cells by RT‐qPCR. ** p < 0.01 and *** p < 0.001 by one‐way ANOVA. d) Relation between CYTOR and FOSL1 mRNA expression in HNSCC based on TCGA database by Pearson correlation analysis. e) Representative images of FOSL1 IHC staining and CYTOR ISH staining in HNSCC tissues. Scale bar, 100 µm. f) Relation between CYTOR and FOSL1 protein expression in HNSCC based on ISH and IHC staining by Pearson correlation analysis. g,h) Kaplan‐Meier curves and log‐rank test for overall survival, g) and disease‐free survival, h) of HNSCC patients grouped by the expression of FOSL1 and CYTOR from SYSU cohort.

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: CYTOR is regulated by FOSL1 in HNSCC. a) FOSL1 occupancies at the locus of CYTOR by FOSL1 ChIP‐seq. b) Knockdown of FOSL1 with siRNA reduced the recruitment of FOSL1 at the promoter of CYTOR in HNSCC cells by ChIP‐qPCR. ** p < 0.01 and *** p < 0.001 by Student's t ‐test. c) Depletion of FOSL1 with siRNA suppressed the expression of CYTOR in HNSCC cells by RT‐qPCR. ** p < 0.01 and *** p < 0.001 by one‐way ANOVA. d) Relation between CYTOR and FOSL1 mRNA expression in HNSCC based on TCGA database by Pearson correlation analysis. e) Representative images of FOSL1 IHC staining and CYTOR ISH staining in HNSCC tissues. Scale bar, 100 µm. f) Relation between CYTOR and FOSL1 protein expression in HNSCC based on ISH and IHC staining by Pearson correlation analysis. g,h) Kaplan‐Meier curves and log‐rank test for overall survival, g) and disease‐free survival, h) of HNSCC patients grouped by the expression of FOSL1 and CYTOR from SYSU cohort.

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: ChIP-sequencing, Knockdown, ChIP-qPCR, Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining

Targeting CYTOR suppresses tumor growth and metastasis in HNSCC. a) Targeting CYTOR with ASO administration by intra‐tumoral injection suppressed HNSCC subcutaneous xenograft growth. b) Quantification of tumor growth curves for HNSCC subcutaneous xenograft with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR administration. *** p < 0.001 by two‐way ANOVA. c) Quantification of tumor weight of HNSCC subcutaneous xenograft. ** p < 0.01 by one‐way ANOVA. d) Representative images of CYTOR ISH staining and FOSL1 IHC staining in HNSCC subcutaneous xenograft treated with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR . Scale bar, 100 µm. e) Targeting CYTOR with ASO systematically administration by tail vein injection inhibited HNSCC orthotopic xenograft growth. f) Representative images of HE staining of SCC1 formed orthotropic xenograft with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR systematically administration. Scale bar, 1 mm. g) Quantification of tumor volume of HNSCC orthotropic xenograft in ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR groups. * p < 0.05 by one‐way ANOVA. h) Representative images of Pan‐CK IHC staining in cervical lymph node from orthotropic tumor bearing mice treated with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR . Scale bar, 300 µm. * p < 0.05 by Chi‐square test. i) Representative images of CYTOR ISH and FOSL1 IHC staining in HNSCC orthotropic xenograft with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR systematically administration. Scale bar, 100 µm.

Journal: Advanced Science

Article Title: CYTOR Facilitates Formation of FOSL1 Phase Separation and Super Enhancers to Drive Metastasis of Tumor Budding Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.1002/advs.202305002

Figure Lengend Snippet: Targeting CYTOR suppresses tumor growth and metastasis in HNSCC. a) Targeting CYTOR with ASO administration by intra‐tumoral injection suppressed HNSCC subcutaneous xenograft growth. b) Quantification of tumor growth curves for HNSCC subcutaneous xenograft with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR administration. *** p < 0.001 by two‐way ANOVA. c) Quantification of tumor weight of HNSCC subcutaneous xenograft. ** p < 0.01 by one‐way ANOVA. d) Representative images of CYTOR ISH staining and FOSL1 IHC staining in HNSCC subcutaneous xenograft treated with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR . Scale bar, 100 µm. e) Targeting CYTOR with ASO systematically administration by tail vein injection inhibited HNSCC orthotopic xenograft growth. f) Representative images of HE staining of SCC1 formed orthotropic xenograft with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR systematically administration. Scale bar, 1 mm. g) Quantification of tumor volume of HNSCC orthotropic xenograft in ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR groups. * p < 0.05 by one‐way ANOVA. h) Representative images of Pan‐CK IHC staining in cervical lymph node from orthotropic tumor bearing mice treated with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR . Scale bar, 300 µm. * p < 0.05 by Chi‐square test. i) Representative images of CYTOR ISH and FOSL1 IHC staining in HNSCC orthotropic xenograft with ASO‐control, ASO1‐ CYTOR , and ASO2‐ CYTOR systematically administration. Scale bar, 100 µm.

Article Snippet: Biotin tagged CYTOR antisense DNA probes were designed and synthesized by LGC Biosearch Technologies.

Techniques: Injection, Control, Staining, Immunohistochemistry